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 Purified Filtrate of Bacillus subtilis natto Culture

 Natto has the highest fibrinolytic activity
 among 200 foods from all over the world.

Natto, which has recently attracted attention throughout the world, is a familiar part of the Japanese diet. Japan has the highest average longevity in the world, which is partly attributed to a high consumption of soybean products, especially "natto". Dr. Sumi, a professor in the Department of Chemical Technology, College of Science and Industrial Technology, Kurashiki University of Science and the Arts, has clarified the beneficial effects of natto. In the US, Dr. Sumi found that the sticky part of natto, commonly called "threads", exhibited a strong fibrinolytic activity. He named the corresponding fibrinolytic enzyme "nattokinase" in 1980.

Dr. Sumi conducted research on about 200 kinds of food from all over the world, and he found that natto had the highest fibrinolytic activity among all those foods. In 1986, Dr. Sumi presented the results of his research in Japan for the first time. Mass media such as NHK and major newspapers were quick to report these results, and natto received much favorable publicity. Since then, sales of natto in Japan have increased dramatically due to its reputation as an essential food for health maintenance.

However, many people in Japan and other countries dislike natto because of the peculiar flavor and odor. For such people, a dried product of natto has been developed, in which the activity of nattokinase remains but the flavor and odor have been removed. This product enables easy oral ingestion of natto for everyone.

There are several proteases in nattokinase (shown in the Table). Although subtilisin protease has long been known to be a nattokinase with an antithrombotic effect, it has become clear that bacillopeptidase F, one of serine proteases, has both the effect of dissolving thrombi and the effect of inhibiting the formation of thrombi.

"NKCP (purified natto culture filtrate)" with bacillopeptidase, developed using an original technique, has been shown to be safe in many studies and is highly expected to prevent thrombosis as "the second generation nattokinase." To guarantee the activity of NKCP, researchers have developed qualitative and quantitative analysis methods to determine the content and activity of bacillopeptidase. Especially, to determine the quantification of bacillopeptidase, they established a sandwich ELISA method.

< Enzymes synthesized and excreted outside the cells by Bacillus subtilis after
exponential multiplication. >
apr Molecular weight by SDS PAGE :20kDa1), 28kDa2) A conventional nattokinase. Its casein decomposing activity and direct fibrinolytic activity have been confirmed. The ability to decompose and inactivate plasminogen activator inhibitor type1 (PAI-1) has also been reported2).
Neutral protease npr Major exopoteinase as with apr
Extracellular protease epr Molecular weight by SDS PAGE: 40-34kDa
Metallo protease mpr Molecular weight by SDS PAGE: 28kDa3)
Bacillopeptidase F bpr Molecular weight by SDS PAGE :47kDa3), 48kDa4), Secreted 92kD protein and converted into 80kDa and 48kDa proteins4). Has high esterase activity as well as proteinase activity5).

1)Sumi et al, Experientia, Vol. 43, 1110-1111, 1987.
2)The Journal of Biological Chemistry, Vol. 276, pp. 24690-24696, 2001.
 Mol Gen Genet 1990 May; 221(3): 486-90
3)Journal of Bacteriology, Vol. 172, pp. 1019-1023, 1990.
4)The Journal of Biological Chemistry, Vol. 265, pp. 6845-6850, 1990.
5)Journal of Bacteriology, Vol. 172, pp. 1470-1477, 1990.

 Scientific data of Nattokinase
  1. Enhancement of the Fibrinolytic Activity in Plasma by Oral Administration of Nattokinase1
  4. Effect of NKCP, a powder produced from dried culture filtrate of partially distilled Bacillus subtilis, on fluidity of blood

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